Jack Jones

October 24, 2022

Sample information
Picture
Photos by: Jack J.
Location
Collection date 12/14/2022
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School
Observations

Female larvae were captured by the Middlesex mosquito commission and then grown into adults before being donated to our class.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit qiagen blood and tissue
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Modification P2 for ATL and N3 for AL

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All procedure was followed correctly and through the gels, A strong band was shown at the 500 mark.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be postive for Wolbachia.
Report Inappropriate Post