Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/20/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | South Plainfield High School |
| Observations | Female mosquito grown by the Middlesex Mosquito Commission and donated to our class. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Culex |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | Modification P2 for ATL and N3 for AL |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | We had no results for the gel showing just the mosquito DNA because it ran off of the gel. In our gel testing for wolbachia DNA, my DNA did not have a band, showing that there is no wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culex was found to be negative for Wolbachia. |
Water Boatman