Mosquito- Avery G

October 24, 2022

Sample information
Picture
Photos by: Avery G
Location
Collection date 09/20/2022
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School
Observations

Female mosquito grown by the Middlesex Mosquito Commission and donated to our class.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Modification P2 for ATL and N3 for AL

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

We had no results for the gel showing just the mosquito DNA because it ran off of the gel. In our gel testing for wolbachia DNA, my DNA did not have a band, showing that there is no wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be negative for Wolbachia.
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