|Captive / Cultivated?||Captive / Cultivated|
|Group||South Plainfield High School|
Female mosquito grown by the Middlesex Mosquito Commission and donated to our class.
|Putative identification||Hexapoda Insecta Diptera Culicidae Culex|
|Extraction kit||DNeasy (Qiagen) blood and tissue kit|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
Modification P2 for ATL and N3 for AL
|Explanation of confidence level||
We had no results for the gel showing just the mosquito DNA because it ran off of the gel. In our gel testing for wolbachia DNA, my DNA did not have a band, showing that there is no wolbachia.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Culex was found to be negative for Wolbachia.|