Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/06/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | Camponotus vicinus Ant. Found under a rock surrounded by dry dirt and leaves. Many ants were found at the time, and we selected one out of the group. Description: Black head and abdomen with a light-colored yellow orange thorax. Body length: 1.2 cm. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus vicinus |
Methods |
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| Extraction kit | Edwards Buffer |
| DNA extraction location | |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | |
| Gel images |
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| Protocol notes | Extraction according to protocol. DNA Elution according to protocol. PCR according to protocol. Gel electrophoresis positive for Arthropod, negative for Wolbachia |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We had a clear band of positive arthropod and no band for Wolbachia. We followed the same procedures and ran the Drosophila (Wolbachia positive and negative) for our controls for comparison and that was a clear result. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | The Camponotus vicinus was found to be negative for Wolbachia. |
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