Camponotus vicinus. Carpenter-like Ant

October 17, 2022

Sample information
Picture
Photos by: Heather A.
Location
Collection date 09/06/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Camponotus vicinus Ant. Found under a rock surrounded by dry dirt and leaves. Many ants were found at the time, and we selected one out of the group.

Description: Black head and abdomen with a light-colored yellow orange thorax.

Body length: 1.2 cm.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus vicinus

Methods
Extraction kit Edwards Buffer
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain
Gel images
Protocol notes

Extraction according to protocol. DNA Elution according to protocol. PCR according to protocol. Gel electrophoresis positive for Arthropod, negative for Wolbachia

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We had a clear band of positive arthropod and no band for Wolbachia. We followed the same procedures and ran the Drosophila (Wolbachia positive and negative) for our controls for comparison and that was a clear result.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Camponotus vicinus was found to be negative for Wolbachia.
Report Inappropriate Post