Sample information
Picture	Photos by: Petrit K.
Location
Collection date	09/14/2022
Captive / Cultivated?	Wild-caught
Group	KantiWil
Observations	I found it also under leaf, birch tree, in a forest
Putative identification	Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniPCR
Buffer	TAE
DNA stain	Fast Blast
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Medium
Explanation of confidence level	on the gel image, were borders
Wolbachia 16S sequence	Download FASTA    Download AB1
Arthropod COI sequence	Download FASTA    Download AB1
Summary	The Drosophila melanogaster was found to be postive for Wolbachia.