lasius flavus AS1

October 7, 2022

Sample information
Picture
Photos by: Annika S, Nikolett L, Janine M
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

Along a path with pebbles, right next to the grass
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius Lasius flavus

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Close to insect line.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GAATAATCATTCGACTAGAATTAGGTTCTTCTAATTCATTAATTAATAATGATCAAATTWATAATTCTATAGTTACAAGACATGCATTCGTTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTTCTCGTACCTTTAATATTAGGATCACCTGATATAGCATACCCCCGTATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATTTCTCTCCTTCTTTTAAGAAATTTTATTAATGACGGAGTAGGAACAGGATGAACTGTTTATCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCCTCAGTTGATTTAACTATTTTTTCTCTTCATATTGCTGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACTATTATAAATATACATCATAAAAATTTTTCTATCGATAAAATTCCCTTACTTGTATGATCAATTTTAATCACTGCAATTTTATTACTTTTATCCCTCCCAGTTCTTGCAGGAGCTATCACTATACTTCTAACTGACCGTAACCTTAATACTTCATTTTTTGACCCATCTGGTGGTGGTGATCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lasius flavus was found to be negative for Wolbachia.
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