Honey Bee

October 13, 2022

Sample information
Picture
Photos by: Brittni R., MaKayla W.
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group Palm Beach Atlantic University
Observations

Already was found dead when caught, body was intact
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Lane 1: DNA Template

Lane 2: Insect- Hymenoptera

Lane 3: (+) control

Lane 4: (-) control

Lane 5: Water

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

In the lane for the insect, a band appeared and matched the negative control. Since this band appeared, it shows that DNA was properly extracted and isolated. There is no band that lines up with the positive control bands. Overall, the confidence level is very high.
Wolbachia 16S sequence
Wolbachia_F (16S_WspecF): 5'-CAT ACC TAT TCG AAG GGA TAG-3' // Wolbachia_R (16S_WspecR): 5'-AGC TTC GAG TGA AAC CAA TTC-3'
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Arthropod_F (LCO1490): 5'GGT CAA CAA ATC ATA AAG ATA TTG G-3' // Arthropod_R (HCO2198): 5'-TAA ACT TCA GGG TGA CCA AAA AAT CA-3'
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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