Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/13/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Palm Beach Atlantic University |
| Observations | Already was found dead when caught, body was intact |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | Lane 1: DNA Template Lane 2: Insect- Hymenoptera Lane 3: (+) control Lane 4: (-) control Lane 5: Water |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | In the lane for the insect, a band appeared and matched the negative control. Since this band appeared, it shows that DNA was properly extracted and isolated. There is no band that lines up with the positive control bands. Overall, the confidence level is very high. |
| Wolbachia 16S sequence |
Wolbachia_F (16S_WspecF): 5'-CAT ACC TAT TCG AAG GGA TAG-3' // Wolbachia_R (16S_WspecR): 5'-AGC TTC GAG TGA AAC CAA TTC-3'
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
Arthropod_F (LCO1490): 5'GGT CAA CAA ATC ATA AAG ATA TTG G-3' // Arthropod_R (HCO2198): 5'-TAA ACT TCA GGG TGA CCA AAA AAT CA-3'
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman