Dark Colored Ants

October 6, 2022

Sample information
Picture
Photos by: Angelina Watai
Location
Collection date 09/27/2022
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations
  • A huge group of ants was going after my dog’s kibble in the bowl.
  • Some ants were carrying kibble crumbs.
  • They appear black but under the microscope, they looked light brown.
  • A pair of antennae and six legs.
  • Had beak-like mouthparts and compound eyes.
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My DNA and negative control were the only ones with bands besides the DNA ladder. The bands were the size detecting arthropod and not Wolbachia too. I expected there to be a band for my positive control but I had no bands for the rest of the samples.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
Report Inappropriate Post