Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/20/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | South Plainfield High School |
| Observations | Female mosquito caputerd and grown into adults by the Middlesex County Mosquito Commission and donated to our class. |
| Putative identification | Arthropoda Insecta Diptera Culicidae Culex |
Methods |
|
| Extraction kit | |
| DNA extraction location | |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | Modification P2 for ATL and N3 for AL |
Results |
|
| Wolbachia presence | |
| Confidence level | |
| Explanation of confidence level | Since our DNA ran off the gel, we had no results to see if they had wolbachia. Testing in our gel for wolbachia, my mosquito DNA had a band showing wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid