RA1 (red ants) Wolbachia Results

October 1, 2022

Sample information
Picture
Photos by: Ashley P
Location
Collection date 09/17/2022
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

Placed a couple pieces of dry cat food on the (screened-in) outdoor kitchen/ lanai floor. Within 24 hrs there were a group of very small red ants surrounding the piece of cat food. There were approximately 10-20 individuals. They moved relatively slowly and without any perceived organization. Within 24-48hrs they would leave that location.

Under the dissecting microscope the small ants appeared to have lighter colored (almost see through) abdomens. With very long legs and antennae, as well as very large mandibles.
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Research project planning

DNA extraction

PCR

Gel electrophoresis

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls worked, no 438bp Wolbachia bands present on the gel for this specimen sample.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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