BA1 (black ant) Wolbachia Results

October 1, 2022

Sample information
Picture
Photos by: Ashley P
Location
Collection date 09/17/2022
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

Ants were observed in somewhat organized lines of two, going up the inside of a table in my screened-in, outdoor kitchen lanai.Upon any disturbance (even air motion) the ants would become scattered and frenzied, but would eventually go back into formation of two lines, all following in the same direction.

Under the dissecting microscope the ants appeared to have lighter segmented abdomens, with unusual white circles showing through the exoskeleton inside their abdomen.
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Research project planning

DNA extraction

PCR

Gel elctrophoresis

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Yes all controls worked and there were 438bp Wolbachia primer bands on the gel for this specimen sample
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be postive for Wolbachia.
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