Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/17/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hawaii Community College |
| Observations | Ants were observed in somewhat organized lines of two, going up the inside of a table in my screened-in, outdoor kitchen lanai.Upon any disturbance (even air motion) the ants would become scattered and frenzied, but would eventually go back into formation of two lines, all following in the same direction. Under the dissecting microscope the ants appeared to have lighter segmented abdomens, with unusual white circles showing through the exoskeleton inside their abdomen. |
| Putative identification | Arthropoda Hexapoda |
Methods |
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| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Research project planning DNA extraction PCR Gel elctrophoresis |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Yes all controls worked and there were 438bp Wolbachia primer bands on the gel for this specimen sample |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hexapoda was found to be postive for Wolbachia. |
Water Boatman