BA1 (black ant) Wolbachia Results

Sample information
Picture	Photos by: Ashley P
Location
Collection date	09/17/2022
Captive / Cultivated?	Wild-caught
Group	Hawaii Community College
Observations	Ants were observed in somewhat organized lines of two, going up the inside of a table in my screened-in, outdoor kitchen lanai.Upon any disturbance (even air motion) the ants would become scattered and frenzied, but would eventually go back into formation of two lines, all following in the same direction. Under the dissecting microscope the ants appeared to have lighter segmented abdomens, with unusual white circles showing through the exoskeleton inside their abdomen.
Putative identification	Arthropoda Hexapoda
Methods
Extraction kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes	Research project planning DNA extraction PCR Gel elctrophoresis
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	Yes all controls worked and there were 438bp Wolbachia primer bands on the gel for this specimen sample
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hexapoda was found to be postive for Wolbachia.

The Wolbachia Project Database