Pill Bug – Henry Terrell

November 6, 2022

Sample information
Picture
Photos by: Henry T
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Living, rolled up into a ball shape, some leg movement, dark-gray color.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am highly confident as all of my controls worked as expected. Additionally, I did not have any problems with the protocol, and I was not surprised by any bands on the gel. Therefore, I am confident my arthropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
BLAST search results didn't match anything.
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidae was found to be negative for Wolbachia.
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