Luca’s Spider

November 11, 2022

Sample information
Picture
Photos by: Dr. O'Brien
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations
Putative identification Arthropoda Chelicerata Arachnida

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The controls worked and the evidence was clear.
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
ATTTAGAGTTTTACTTCCCCCNNNTTAAGACAGCCTGGAACATATTTAGGGGAAGATCCTCTCTATAATGTTATCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTTTAATTGGGGGTTTTGAGAATTGATTAGTACCTTTAATATTGGGGGCGCCTGATATAGCTTTTCCACNAATAAATAATTTNAGATTTTGGTTATTACCACCTTCTTTGTTTTTATTATTTATTTCTTCTATGGCTGAGATAGGGGTTGGAGCAGGTTGAACTGTGTATCCTCCCTTAGCTTCATCTGTTGGTCATGCTGGAAGTGCGGTGGATTTTGCTATTTTTTCTTTACATTTGGCTGGTGCTTCTTCTATTATAGGGGCTATTAATTTTATTTCTACTATTTTTAATATACGTTCTTATGGGATATCTATAGAGAAAGTTCCTTTGTTTGTTTGATCGGTTTTAATTACTGCTATTTTATTATTATTGTCTTTACCCGTGTTAGCTGGGGCTATTACTATATTGTTAACTGATCGGAATTTTAATACATCTTTTTTTGATCCAGCANGGGGTGNAGATCCAATTTTATTTCAACATTTGTTTTGATTTTTTGGTCACCCTGGAAGTTNNA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arachnida was found to be postive for Wolbachia.
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