Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | |
| Group | St. Albans School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am confident that my beetle did not have Wolbachia. I followed the protocol at all times to ensure the most accurate results. Additionally, my professor backchecked our procedures and gels to confirm. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
NNGGATTACTTCCTCCTTCTATTTCATTACTATTAGCTAGAAGATTTATTGAGTCAGGGGCGGGAACGGATGAACAGTTTACCCTCCTCTTTCCAGTAATATTGCCCACAGTGGAGCCTCAGTAGATTTAACAATTTTTAGCCTTCATTTGGCAGGAATCTCTTCAATTTTGGGGGCAGTTAATTTCTTCAATTATAAATATACGAACCCCTGGAATAACAATAGAAAAAACCTTTATTTGCTTGATCTGAGGAATTACTGCCGTTCTATTACTTCTTTCTCTCCCAGCAGGATACATACTTTTAACGATGAATTTAATTTTCGGGGAGACTTTTATTTTGTTGGCANT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Harmonia Axyridis (Asian Lady Beetle)
(no title)
Brown Marmorated Stink Bug Wolbachia Project
Asian Lady Beetle Wolbachia Project
Melanoplus sp.