Horned Passalus (Bess Beetle)

Sample information

Photos by: Photo by Zak I.
Collection date
Captive / Cultivated?
Group St. Albans School
Putative identification Arthropoda Hexapoda Insecta Coleoptera


Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident that my beetle did not have Wolbachia. I followed the protocol at all times to ensure the most accurate results. Additionally, my professor backchecked our procedures and gels to confirm.

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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