Horned Passalus (Bess Beetle)

November 10, 2022

Sample information
Picture
Photos by: Photo by Zak I.
Location
Collection date
Captive / Cultivated?
Group St. Albans School
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident that my beetle did not have Wolbachia. I followed the protocol at all times to ensure the most accurate results. Additionally, my professor backchecked our procedures and gels to confirm.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
NNGGATTACTTCCTCCTTCTATTTCATTACTATTAGCTAGAAGATTTATTGAGTCAGGGGCGGGAACGGATGAACAGTTTACCCTCCTCTTTCCAGTAATATTGCCCACAGTGGAGCCTCAGTAGATTTAACAATTTTTAGCCTTCATTTGGCAGGAATCTCTTCAATTTTGGGGGCAGTTAATTTCTTCAATTATAAATATACGAACCCCTGGAATAACAATAGAAAAAACCTTTATTTGCTTGATCTGAGGAATTACTGCCGTTCTATTACTTCTTTCTCTCCCAGCAGGATACATACTTTTAACGATGAATTTAATTTTCGGGGAGACTTTTATTTTGTTGGCANT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
Report Inappropriate Post