Ant

November 21, 2022

Sample information
Picture
Photos by: Pan L.
Location
Collection date 09/10/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Captured underneath a moist leaf pile. No other ants were spotted nearby. Caught around 5pm in the afternoon and immediately put under refrigeration after photography. 6 legs, head/abdomen/thorax insect.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

A strong positive band was revealed for the ant, but none of the other 3 arthropods. No noticeable errors were made during electrophoresis.

We used a 10kb DNA ladder

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

No errors were made noticeable during or after the procedure.
Wolbachia 16S sequence Download FASTA    Download AB1
>Mod B1-WSPECF.scf (352 bp) GGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGT AATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATG GAGTGGGCTACACACGTGCTACAATGGTGGCTATAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCAT CTCANTTCGGATTGTACTCTGCAACTCNAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAA TACGTTCTCGGGTCTTGTACACACTGCCCGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>Mod B1-CO1F.scf (592 bp) AATTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTAGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCA ATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTT TTAATTCCACTTATACTAGGATCTCCTGATATAGCTTACCCTCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATC GATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGAACTATCTACCCCCCTCTATCATCAA ATACCTTCCATAGNGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCA ATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTANTATGATCTAT TCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGCGCTATTACAATACTACTAACAGACCGAAATC TTAATACTTCATTTTTCGATCCCTCGGGAGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be postive for Wolbachia.
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