Bald-Faced Hornet

November 21, 2022

Sample information
Picture
Photos by: Pan L.
Location
Collection date 09/20/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Captured on top of a moist leaf pile. No other hornets were spotted nearby. Caught around 5pm in the afternoon and immediately put under refrigeration after photography. 6 legs, head/abdomen/thorax insect.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

No band was revealed for the hornet, along with none of the Mantis and fly. The ant was positive. No noticeable errors were made during electrophoresis.

We used a 10kb DNA ladder

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No errors were made noticeable during or after the procedure.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
>Mod B2-CO1F.scf (594 bp) TTAGGAGCCTCAATAAGAATAATTATTCGAATAGAATTGAGATCTCCAGGAGCATTAATTAATAATGATCAAATTTATAA TACAATTATTACAGCTCATGCATTTGTTATAATTTTTTTTATAGTTATACCTTTTATAATTGGAGGATTTGGAAATTGAT TAATTCCTATAATACTAGGTGTTCCAGATATAGCTTTCCCACGAATAAACAATATAAGATTTTGACTCCTTCCACCATCA TTATTTCTTTTAATTAGAANAAGATTTATTAGTGGAGGTGTAGGAACAGGTTGAACCTTATATCCACCTCTTTCTTCTCT TATTGGACATAATTCTCCCTCAGTTGATTTAAGAATTTTTTCAATCCATATTGCTGGAATTTCATCAATTATAGGATCTA TTAATTTTATTGTAACAATTTTAAATATACATACAAAAACTAATTCATTAAATTTTTTACCTTTATTTTCATGATCAGTT TTAATTACTNCTATTCTTTTAATTTTATCATTACCAGTTTTANCTGGTGCAATTACTATATTATTATCTGATCGAAACTT TAATACATCATTTTTTGATCCTACAGGAGGAGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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