Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/20/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Captured on top of a moist leaf pile. No other hornets were spotted nearby. Caught around 5pm in the afternoon and immediately put under refrigeration after photography. 6 legs, head/abdomen/thorax insect. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | No band was revealed for the hornet, along with none of the Mantis and fly. The ant was positive. No noticeable errors were made during electrophoresis. We used a 10kb DNA ladder |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | No errors were made noticeable during or after the procedure. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
>Mod B2-CO1F.scf (594 bp) TTAGGAGCCTCAATAAGAATAATTATTCGAATAGAATTGAGATCTCCAGGAGCATTAATTAATAATGATCAAATTTATAA TACAATTATTACAGCTCATGCATTTGTTATAATTTTTTTTATAGTTATACCTTTTATAATTGGAGGATTTGGAAATTGAT TAATTCCTATAATACTAGGTGTTCCAGATATAGCTTTCCCACGAATAAACAATATAAGATTTTGACTCCTTCCACCATCA TTATTTCTTTTAATTAGAANAAGATTTATTAGTGGAGGTGTAGGAACAGGTTGAACCTTATATCCACCTCTTTCTTCTCT TATTGGACATAATTCTCCCTCAGTTGATTTAAGAATTTTTTCAATCCATATTGCTGGAATTTCATCAATTATAGGATCTA TTAATTTTATTGTAACAATTTTAAATATACATACAAAAACTAATTCATTAAATTTTTTACCTTTATTTTCATGATCAGTT TTAATTACTNCTATTCTTTTAATTTTATCATTACCAGTTTTANCTGGTGCAATTACTATATTATTATCTGATCGAAACTT TAATACATCATTTTTTGATCCTACAGGAGGAGGA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman