Dobsonfly

November 21, 2022

Sample information
Picture
Photos by: Lucy K., Stella T.
Location
Collection date 07/21/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

The dobsonfly was caught during the day between 80-90 degree Fahrenheit weather.
Putative identification Arthropoda Hexapoda Insecta Megaloptera Corydalidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction: The dobsonfly’s abdomen was extracted to use for the DNA sample.

Gel electrophoresis lanes:

Lane 1: 10kb DNA Ladder
Lane 2: Arthropod Sample A-1
Lane 3: Arthropod Sample A-2 (the dobsonfly)
Lane 4: Arthropod Sample A-3
Lane 5: Arthropod Sample A-4
Lane 6: Positive Arthropod Control
Lane 7: Negative Arthropod Control
Lane 8: Positive DNA Control
Lane 9: Water

Analysis:

Sample A-2 tested negative for arthropod DNA, which means an error occurred with that DNA sample somewhere in this process or one of the earlier processes. We know we can trust our results have no contamination because our controls tested correctly.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

Since the dobsonfly sample tested negative for arthropod DNA, it is unknown whether or not the dobsonfly is infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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