Red Imported Fire Ant

December 13, 2022

Sample information
Picture
Photos by: Carley. V, Maggie. C, Paloma. C, Gabby. S
Location
Collection date 12/02/2022
Captive / Cultivated? Wild-caught
Group Wheeler High School
Observations

We found this ant in a small ant hill on the grassy area of Wheeler High School. We collected the ant in the early afternoon, and the temperature was about 60 degrees Fahrenheit. There were many ants, but we only collected about 7.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Gel Electrophoresis Lanes-

Most Right Lane= Lane 1

1- Ant that focused on in this post (Circled in Red on Gel)

2- Other ant (not covered in this post)

3- Other ant (not covered in this post)

4- Positive Arthropod Control

5- Negative Arthropod Control

6- Positive DNA Control

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of our controls worked as expected. We did not have any problems with my protocol, and the bands on the gel were extremely clear. Therefore, we are very confident that our arthropod is Wolbachia negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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