Misumena vatia- Small crab like Spider.

March 11, 2023

Sample information
Picture
Photos by: Chase Hartzell, Heather Ahlstrom
Location
Collection date 09/06/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations
  1. Small crab like Spider. Found on web attached to a small green brush plant.

Description: Pale translucent yellow with long front legs  shaping it like crab legs.

Body length: .25 cm. Found by Alex Hartzell.
Putative identification Arthropoda Chelicerata Arachnida Araneae Thomisidae Misumena Misumena vatia

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Used the Wolbachia Primers and Protocols
Wolbachia 16S sequence
Arthropod COI sequence
>CO1_4_CspecF GAGCTTGATCAGCTATAGTTGGTACTGCTATAAGAGTTTTAATTCGGATGGAGTTAGGTAATTCTGGAAGATTATTAGGAAATGATCATTTATATAATGTTATTGTGACTGCTCATGCTTTTGTAATAATTTTTTTTATAGTAATACCAATTTTGATTGGAGGTTTTGGTAATTGATTAGTACCTTTAATATTAGGAGCTCCTGATATATCTTTTCCACGTATAAATAATTTATCTTTTTGGTTGTTACCCCCTTCATTGTTTTTATTATTTATATCTTCTATGGTTGAAATAGGGGTTGGTGCGGGTTGAACAGTATACCCTCCTTTAGCTTCTAGAGTAGGTCATATAGGAAGATCAATGGATTTTGCTATTTTTTCTCTTCATTTAGCTGGTGCTTCATCAATTATAGGAGCTGTTAATTTTATTTCTACAATTATTAATATACGGAGTGAGGGGATAAGAATAGAAAAAGTTCCTTTATTTGTTTGATCTGTTTTAATTACAGCTATTTTATTGCTTTTATCGCTTCCTGTTTTAGCAGGAGCTATTACTATATTATTGACTGATCGTAATTTTAATACTTCTTTTTTTGACCCGGCTGGGGGAGGGGATCCAATTTTATTTCAACATTTGTTTTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Misumena vatia was found to be negative for Wolbachia.
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