Podalonia robusta- Red tailed wasp

March 11, 2023

Sample information
Picture
Photos by: Chase Hartzell, Heather Ahlstrom
Location
Collection date 09/06/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Podalonia robusta- Red tailed wasp- on a light purple flower

Description: yellow and black body with very thin connector between thorax and abdomen

Body length: 1.52 cm. Found by Heather Ahlstrom
Putative identification Arthropoda Insecta Hymenoptera Sphecidae Podalonia Podalonia robusta

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Project PCR Primers for both Wolbachia and CO1 Arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
>CO1_9_CspecF GAGCAGGAATATATGGAGCTTCATTAAGTATAATTATTCGAATAGAATTAAGAATCCCAGGAAAATTAATTGGAAATGATCAAATTTATAATAGTATTGTTACTGCACATGCTTTTATTATAATTTTTTTTATAATTATACCATTTATAATTGGAGGATTCGGAAATTGATTAATTCCTCTAATACTAGGAGCACCAGATATAGCTTACCCTCGAATAAATAATATAAGATTCTGACTTTTACCGCCATCTTTACTTTTATTAATCTCTAGAAGAATTATTGACTCTGGAACAGGAACTGGTTGAACAGTTTATCCACCCTTATCTTCTAATATATTTCATAATGGATTATCAGTTGATTTTAGAATTTTATCTTTACACCTAGCTGGTGCGTCTTCCTTAATAGGATCTATCAACTTTATTTCAACTATTTTTAATATAAAAAATATTAATCTATCTATAAATAAAATTTCATTATTTACATGATCAATTTTAATTACAACATTCTTATTACTTTTATCTTTACCAGTCCTAGCAGGAGCAATTACTATGCTATTAACTGATCGAAACCTAAATACATCATTTTTCGATCCAACAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Podalonia robusta was found to be negative for Wolbachia.
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