NESACBsp23-23

April 10, 2023

Sample information
Picture
Photos by: Jordon S
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

1/23/23, 1:23 P.M., Ant hill near aquaponics center, GSU Armstrong. Grabbed with forceps after turning over dirt.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Based on the controls used in this experiment I can say with high confidence that there is no Wolbachia present in my insect sample.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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