NESACBSP23BM12 coleoptera

Sample information

Picture
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found on a leaf in the parking lot just sitting there peacefully.

Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods

Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

I completed every step correctly and had my instructor and lab partner check behind me.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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