Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/26/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | •Environment- Under wet bed of leaves on top of soil
•Weather- Cold, sunny and wet
•Method of collection- Digging in dirt
|
| Putative identification | Arthropoda Insecta Blattodea |
Methods |
|
| Extraction kit | |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I have high confidence my insect has Wolbachia based off of my gel electrophoresis results. There was a clear band for both my insect and positive Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Blattodea was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid