NESACKSp23-24-MM Hymenoptera

April 13, 2023

Sample information
Picture
Photos by: Mae Jeuel M
Location No map location data available.
Collection date 01/26/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Location: The Pond(North)
Date:01/26/23
Time: Morning 10:00 am
Weather: Sunny/Windy/Cold 48 degrees
Habitat: Wet Area Near Edge Of Pond
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am pretty confident that my insect results coming back negative are correct due to the carefulness of my partner and me during the extraction process.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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