Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/26/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Found near a large point under a fallen, rotting tree branch that was slightly damp. |
| Putative identification | Arthropoda Hexapoda Insecta Isoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I did need some help, but I was successfully able to extract DNA from my insect and proceed with the gel process. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Isoptera was found to be negative for Wolbachia. |
Water Boatman