GREACFSP23-02 Hymenoptera

April 19, 2023

Sample information
Picture
Photos by: Keyshauna D.
Location
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time of collection: 1:21pm

Location: in front of Jenkins Hall in the shrubs

Environment of Collection: Gloomy about 72 degrees

Method of collection: Sweeping Net
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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