GRE-ACF-Sp23-15 Coleoptera

April 25, 2023

Sample information
Picture
Location
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Collection location: greenhouse
  • Collection method: beat stick and net
  • Collection habitat: bushes, mulch
  • Weather conditions: sunny
  • Multiple of the same type of insects were in the area
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confidence that my insect is negative for Wolbachia because Wolbachia 16S was not found present in this insect’s DNA that we tested through a gel electrophoresis system.  Meaning the only present band was the CO1 band and not the Wolbachia 16S rRNA band.
Wolbachia 16S sequence
Arthropod COI sequence
TGAGCAGGTATAATTGGAACATCCCTAAGAATTCTAATTCGAGCAGAATtacgttgacCCGGTTCCTTAATTGGTAATGATCAAATTTATAatcttatTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGCGGATTTGGTAACTGACTAGTCCCATTAATAATTGGAGCCCCTGATATAGCATTCCCTCGAATAAATAATATAAGATTTTGACTTTTACCACCCTCTTTATTATTATTATTATTAAGAAGAATAGTAGAAAATGGAGTTGGTACCGGATGAACAGTTTATCCCCCTTTATCATCCAATATTGCTCACGGAGGTTCATCAGTTGATTTAGCTATTTTTAGTTTACACTTGGCTGGAATTTCTTCAATTTTAGGAGCAATTAATTTTATTACTACAGTAATTAATATACGTCCAATAGGAATAACTTTTGACCGAATACCATTATTTGTATGAGCAGTTGTTATTACAGCAGTTTTACTACTATTATCATTACCTGTTCTTGCCGGAGCAATTACAATATTATTAACAGATCGTAATCTAAATACCTCTTTTTTTGATCCTGCTGGAGGTGGTGATCCTATTTTATATCAACATTTATTTTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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