Moth – AL040623

May 15, 2023

Sample information
Picture
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations
  • The moth was flying when we caught it
  • The moth was a brown color
  • It was flying around the school garden
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  • Lane 1 – DNA Ladder
  • Lane 2 – Arthropod 1 (Wood bug)
  • Lane 3 – Arthropod 2 (Moth)
  • Lane 4 – Arthropod Positive Control
  • Lane 5 – Arthropod Negative Control
  • Lane 6 – DNA Positive Control
  • Lane 7 – Water

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Our confidence level is low because none of the bands showed up except for our DNA ladder and DNA control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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