Sample information
Picture	Photos by: Adam Chen
Location
Collection date	04/14/2023
Captive / Cultivated?	Wild-caught
Group	Arroyo High School
Observations
Putative identification	Arthropoda Hexapoda Insecta Diptera Psychodidae
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Rear half
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Psychodidae was found to be negative for Wolbachia.