Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/07/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Thrive Scholars |
| Observations | |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | We followed the protocol on the website. Top gel is Arthropod gel. Bottom gel is Wolbachia gel. Sample is in Lane two of gel one and lane four of gel two We might have swapped the samples.
|
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | We suspect that the control samples were swapped which caused the bands to be off. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Spotted cucumber beetle (Diabrotica undecimpunctata) C-5 SSP
DKH10
DKH 9
DKH8
DKH 7