Thrive Scholars – Summer 2023 – Pillbug – Easties

Sample information

Collection date 08/08/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae


Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

We followed the protocol on the website.

the top gel is wolbachia

bottom gel is arthropod

The sample is in second lane of both gels


Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The controls did not work, no Wolbachia was detected. Although it was Arthopod Postitive.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be negative for Wolbachia.
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