Portland State University – Chlorochroa sp.

August 18, 2023

Sample information
Picture
Photos by: Rebecca W.
Location
Collection date 08/10/2023
Captive / Cultivated? Wild-caught
Group Portland State University
Observations

This insect was found on a milkweed plant in an urban garden.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Pentatomidae Chlorochroa

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

We use GoTaq Green Master Mix, 1% agarose gel, 1% TAE buffer, and 95 V for one hour.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

This was part of a protocol test. I extracted the DNA from this stink bug on the same day with the same protocol as two positive control and two negative control Drosophila supplied by your project. I dissected two pieces of abdomen from the stink bug and ran through the extraction protocol with both. Neither of the stink bug DNA extracts gave positive results for Wolbachia while both of the positive control Drosophila did.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Chlorochroa was found to be negative for Wolbachia.
Report Inappropriate Post