cranefly

September 7, 2023

Sample information
Picture
Photos by: Mamana V, Lisa K
Location
Collection date 04/05/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

-On the wall

-It was warm outside with a cool breeze

-It wasn’t moving

-It was going crazy inside our container
Putative identification Arthropoda Hexapoda Insecta Diptera Tipulidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Our confidence level is medium because we know that our controls were working but we don’t know if we can trust the results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tipulidae was found to be negative for Wolbachia.
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