Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/05/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | -On the wall -It was warm outside with a cool breeze -It wasn’t moving -It was going crazy inside our container |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Tipulidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Our confidence level is medium because we know that our controls were working but we don’t know if we can trust the results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Tipulidae was found to be negative for Wolbachia. |
Water Boatman