Sample information |
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Picture |
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Location | |
Collection date | 11/02/2023 |
Captive / Cultivated? | Wild-caught |
Group | Antioch High School |
Observations | I caught it around the school i was walking out and i was looking around for some arthropod and i saw him jumping so i caught him |
Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
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Extraction kit | Wizard SV (Promega) |
DNA extraction location | Rear half |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TBE |
DNA stain | SYBR Safe |
Gel images |
|
Protocol notes | when i was smashed the bug in the tube it was turn green. when i start to spin it it would be skin and the DNA was thick. |
Results |
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Wolbachia presence | Unknown |
Confidence level | Low |
Explanation of confidence level | i think mine does have some what Wolbachia because i did some research and it said half of the population of grasshopper has Wolbachia. i said my confidence level is low because we used the same tip when getting the dna. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary |