Sample information |
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Picture |
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Location | |
Collection date | 09/18/2023 |
Captive / Cultivated? | Wild-caught |
Group | Antioch High School |
Observations | The only thing that changed in the insect was that the orange color of its body was removed a little. |
Putative identification | Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus |
Methods |
|
Extraction kit | promega wizard SV |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | |
Buffer | TBE |
DNA stain | SYBR Safe |
Gel images |
|
Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | Low |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Acheta domesticus was found to be negative for Wolbachia. |