Sample information |
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Picture |
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Location | |
Collection date | 09/08/2023 |
Captive / Cultivated? | Wild-caught |
Group | Antioch High School |
Observations | I caught this arthropod at the Antioch High School baseball field. I collected it at about 3:00 pm, it was 82 degrees and sunny. This was the only spider I saw in the area. |
Putative identification | Arthropoda Chelicerata Arachnida Araneae Theridiidae Parasteatoda |
Methods |
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Extraction kit | Wizard SV (Promega) |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | |
Buffer | |
DNA stain | |
Gel images |
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Protocol notes | We had to freeze our samples and start again another day midway into the lab. |
Results |
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Wolbachia presence | |
Confidence level | |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary |