Periplaneta americana

November 20, 2023

Sample information
Picture
Location
Collection date 10/10/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Fall, Urban, hot temp

Brown, 6 legs with hairs on the back pair. Semi translucent shell, 2 antennae, armored head.
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no band for Wolbachia under E3, and there was a strong band under the positive control. Researchers at Penn State sent DNA sequences that matched the visual identification.
Wolbachia 16S sequence Download AB1
Arthropod COI sequence Download AB1
Summary The Blattodea was found to be negative for Wolbachia.
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