Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/12/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Large black eyes, spiral mouthpiece, 6 legs, 2 feeler-things, thin paper wings, spikes on legs, hairy abdomen. |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There was no band under E4, but there was a band under the Wolbachia positive control. Penn State professionals sent us a sequence that matched the visual identification of E4, confirming it as a Pale Mottled Willow. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Water Boatman