Caradrina clavipalpis

November 20, 2023

Sample information
Picture
Location
Collection date 10/12/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Large black eyes, spiral mouthpiece, 6 legs, 2 feeler-things, thin paper wings, spikes on legs, hairy abdomen.
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no band under E4, but there was a band under the Wolbachia positive control. Penn State professionals sent us a sequence that matched the visual identification of E4, confirming it as a Pale Mottled Willow.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Lepidoptera was found to be negative for Wolbachia.
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