|Captive / Cultivated?||Wild-caught|
|Group||Edmund Burke School|
Large black eyes, spiral mouthpiece, 6 legs, 2 feeler-things, thin paper wings, spikes on legs, hairy abdomen.
|Putative identification||Arthropoda Hexapoda Insecta Lepidoptera|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
|Explanation of confidence level||
There was no band under E4, but there was a band under the Wolbachia positive control. Penn State professionals sent us a sequence that matched the visual identification of E4, confirming it as a Pale Mottled Willow.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download AB1
|Summary||The Lepidoptera was found to be negative for Wolbachia.|