Small Ant

March 15, 2024

Sample information
Picture
Location
Collection date 08/29/2023
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Dark brown, small, in a cluster with others of its kind, on a concrete sidewalk near grass.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes
  1. Pure arthropod DNA was extracted and simplified, then was placed in 2 microliters of template DNA.
  2. 23 microliters of PCR cocktail was added with the following components: arthropod_F primer, arthropod_R primer, water, and Taq master mix (5X)
  3. Tube was run in the standard Taq protocol

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Bands in gel were very clear and easy to see, meaning that DNA extraction went perfectly and was done correctly. This likely means that the DNA is reliable and accurate.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
Report Inappropriate Post