Small Ant

Sample information

Collection date 08/29/2023
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School

Dark brown, small, in a cluster with others of its kind, on a concrete sidewalk near grass.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes
  1. Pure arthropod DNA was extracted and simplified, then was placed in 2 microliters of template DNA.
  2. 23 microliters of PCR cocktail was added with the following components: arthropod_F primer, arthropod_R primer, water, and Taq master mix (5X)
  3. Tube was run in the standard Taq protocol


Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Bands in gel were very clear and easy to see, meaning that DNA extraction went perfectly and was done correctly. This likely means that the DNA is reliable and accurate.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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