Small Black Ant

Sample information

Photos by: Trevor H
Collection date 08/29/2023
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School

The arthropod appears to be an ant with six legs and three body parts. The arthropod was found next to a grassy field next to an ant nest. The arthropod was collected during summer, around noon, in 85F sunny weather.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The result of the gel electrophoresis is in lane 5 from left to right.


Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked, and the gel electrophoresis results are clearly visible. There is only one band, meaning there is no Wolbachia DNA present.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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