Allonemobius fasciatus

November 20, 2023

Sample information
Picture
Photos by: Keazia H.G
Location
Collection date 09/12/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations
  • Moves by hoping
  • has significantly long legs
  • long body
  • Green color
  • 6 legs
  • seems to make a creaking noise
  • caught during the fall
  • urban area
  • Humid and hot
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA extraction: It took a while to grind and there was big chunks of the arthropod that needed to be grinder up for extra time. No mistakes were made.

PCR: No mistakes were made.

Gel electrophoresis, Arthropod CO1: Each lane of our gel were positive for an arthropod gene except the water. As for the wolbachia gene it was present in arthropod C1 only and with the positive wolbachia control. No mistakes were made and controls worked fine.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Confidence level is medium  because we took precaution with all our our steps and no mistakes or contaminations were made and everything went smoothly but at the same time the wolbachia band was faint within this arthropod so there is a low chance it is infected.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Query 1 TTTATAACGTTATTGTAACAGCCCACGCATTTGTAATAATTTTCTTTATAGTTATACCCA 60 |||||||||| |||||||||||||||||||||||||||||||| |||||||||||||||| Sbjct 111 TTTATAACGTCATTGTAACAGCCCACGCATTTGTAATAATTTTTTTTATAGTTATACCCA 170 Query 61 TTATAATTGGGGGNNNCGGAAATTGATTAGTACCTTTAATGTTATCAGCCCCTGATATAG 120 | |||||||| || |||||||||||||||||||||||||||||||||||||||||||| Sbjct 171 TCATAATTGGAGGATTCGGAAATTGATTAGTACCTTTAATGTTATCAGCCCCTGATATAG 230 Query 121 CATTCCCCCGAATAAATAATATAAGATTTTGGCTTCTTCCTCCATCATTAACTTTACTTT 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 231 CATTCCCCCGAATAAATAATATAAGATTTTGGCTTCTTCCTCCATCATTAACTTTACTTT 290 Query 181 TATCTAGAAGAATAGTAGAAAATGGAGCAGGAACTGGTTGAACAGTATACCCTCCTTTAT 240 ||||||||||||||||||||||||||||||| ||||||||||||||||||||||| |||| Sbjct 291 TATCTAGAAGAATAGTAGAAAATGGAGCAGGTACTGGTTGAACAGTATACCCTCCCTTAT 350 Query 241 CTTCCTTAATTGCTCACGGAGGAGCATCNGTANATTTAGCTATCTTCTCCCTACACCTAG 300 |||||||||||||||||||||||||||| ||| ||||||||||||||||||||||||||| Sbjct 351 CTTCCTTAATTGCTCACGGAGGAGCATCCGTAGATTTAGCTATCTTCTCCCTACACCTAG 410 Query 301 CAGGAATTTCCTCTATTTTAGGAGCAATTAACTTTATTTCAACNNNNATTAATATACNAG 360 ||||||||||||||||||||||||||||||||||||||||||| |||||||||| || Sbjct 411 CAGGAATTTCCTCTATTTTAGGAGCAATTAACTTTATTTCAACTATAATTAATATACGAG 470 Query 361 CTCCCGGCATAACTCTANNNNNNACCCNNNNNNTTGTGNNNNNNGTANGAATNNNNGCCC 420 ||||||||||||||||| |||| ||||| ||| | || |||| Sbjct 471 CTCCCGGCATAACTCTAGACCAAACCCCCCTATTTGTGTGAGCAGTAGGGATCACTGCCC 530 Query 421 TCCTTCNTCNTCTTTCATTGCCNGTTCTTGCNNGNGCTATTACNNNACTTTTANCAGATC 480 |||||| || ||||||||| || |||||||| | |||||||| ||||||| |||||| Sbjct 531 TCCTTCTTCTTCTTTCATTACCTGTTCTTGCAGGTGCTATTACCATACTTTTAACAGATC 590 Query 481 NAANTTNANACACNTCANTTTTTG 504 || || | |||| ||| |||||| Sbjct 591 GAAATTTAAACACATCATTTTTTG 614
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
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