BRO_ACC_ F23_ACW

November 6, 2023

Sample information
Picture
Photos by: Anna W
Location No map location data available.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught in tall grasses on GSU campus with a sweep net.
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Due to the fact that my controls and my CO1 gene were very visible, I am confident that the lack of light on my 16s specimen well proves that there was no Wolbachia in my insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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