Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/21/2023 |
| Captive / Cultivated? | |
| Group | Georgia Southern University |
| Observations | We used a sweep net to catch our insects. The insects were located at the GSU Armstrong Campus on the north end by the garden. |
| Putative identification | Arthropoda Hexapoda |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | I feel pretty confident with the material in this class |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hexapoda was found to be postive for Wolbachia. |
Water Boatman