MACACDF23-6 Hymenoptera

November 7, 2023

Sample information
Picture
Photos by: Danielle B.
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The collection habitat for my insect was in a woody area on a sunny day. The method used was a sweep net.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I felt confident throughout this entire process because my instructor gave me plenty of opportunities to practice with the different equipment used in this research project such as loading the gel in the wells, pipetting the correct volumes and etc. She also corrected me on any errors before moving forward to the next step.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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