Horned Passalus Beetle

November 7, 2023

Sample information
Picture
Photos by: Madeline D
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations
  • Horned Passalus Beetle was located under a rotting log in Long Hunter State Park
  • Beetle was identified as an arthropod
  • Beetle had a thick black exoskeleton
  • Beetle was approximately 2 centimeters long
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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