Horned Passalus Beetle

November 7, 2023

Sample information
Picture
Photos by: Madeline D
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations
  • Horned Passalus Beetle was located under a rotting log in Long Hunter State Park
  • Beetle was identified as an arthropod
  • Beetle had a thick black exoskeleton
  • Beetle was approximately 2 centimeters long
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes
  • I know for sure that some of my measurements were inaccurate when working with the micropipette.
  • Due to this, I expect for there to be several inaccuracies in the results of my tests.
  • Besides that, the rest of my testing ran smoothly

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

All of the controls worked as expected with the exception of the (-) Arthropod Control. On this sample, there was no trace of Wolbachia or insect DNA, meaning that something went wrong when we were extracting the DNA. Other than that, all of the other samples ran as expected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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