Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/22/2023 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Georgia Southern University |
| Observations | Collection method: sweep net Environment: tall grassy area behind the greenhouse by the Aquaponics center |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | none |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I choose medium because i feel like my pipetting wasn’t the best and if I properly did it correctly the results may be different or it may be the same. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Water Boatman