LOGACF-23-KH

November 7, 2023

Sample information
Picture
Photos by: Kara H.
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

long hind legs built for jumping. caught in grassy lawn area. 95 degrees and sunny with high humidity.
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit none
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

My confidence went down when I found out the Wolbachia prevalence was unknown/undefined because it makes me think I’ve made a mistake throughout the experiment. I was confident it would not be infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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