LOGACEF23-AT Hemiptera

November 7, 2023

Sample information
Picture
Photos by: Angela T.
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Georgia Southern University- Armstrong Science Center, low grass, 84 degrees, humid & sunny.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The positive control yielded positive results. The negative control yielded negative results. The non-template control yielded no results. The results from gel electrophoresis show bright base pairs for both the positive and negative lines.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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