Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/22/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Georgia Southern University- Armstrong Science Center, low grass, 84 degrees, humid & sunny. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The positive control yielded positive results. The negative control yielded negative results. The non-template control yielded no results. The results from gel electrophoresis show bright base pairs for both the positive and negative lines. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be postive for Wolbachia. |
Water Boatman