MACACIF23-8; Hymenoptera

November 8, 2023

Sample information
Picture
Photos by: Samuel M
Location
Collection date 08/08/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Specimen was collected in front of the student union underneath a tree at the main intersection of the sidewalk. It was a bright summer day, partly cloudy w/o precipitation, temperature was a mild 87 degrees. The habitat of the insect was in the dirt under moderate foliage.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls were set up appropriately with no false negatives or positive along with no bands appearing in the NTC. When comparing my result with the Negative control, they appeared identical.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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