BROACHF-23-LW1 Hempitera

November 8, 2023

Sample information
Picture
Photos by: Lauren, W.
Location No map location data available.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • The method used was sweeping net
  • Instead of going side-to-side, we decided to drag it through the grass
  • Grassy tree area near the North End of Georgia Southern University Armstrong Campus
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

There was a high prevalence of Wolbachia in my insect order, so I was confident that my insect would have Wolbachia. Positive, negative and no template control (NTC)came out as expected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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