E1

December 4, 2023

Sample information
Picture
Location
Collection date 10/13/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Controls worked, clearly had no band, only appeared positive in C01.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Drosophila melanogaster was found to be negative for Wolbachia.
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